Effects of Bacterial-Feeding Nematodes and Glucose on Phenanthrene Removal by Pseudomonas putida

摘要

Bacterial-feeding nematodes can promote the bacterial activity through feeding.Bacterial abundance and their activity affect the degradation of polycyclic aromatic hydrocarbons(PAH) such as phenanthrene.The effects of bacterial-feeding nematodes,bacteria,and their interactions on the degradation of phenanthrene with or without glucose were studied through a microcosm experiment.The results showed that up to 57.0%of phenanthrene in mineral medium contaminated with phenanthrene was degraded in the control with bacteria alone and bacteria with the presence of nematodes and/or glucose increased the degradation of phenanthrene by 25.6%to 36.6%.Although both nematode and bacteria abundance decreased gradually,catechol 2,3-dioxygenase(C230) activity increased during the incubation period.Compared with bacteria alone,the presence of nematodes significantly increased C230 activity as well as the abundance of bacteria;this effect was more pronounced when glucose was present.The results imply that nematodes might promote the removal of phenanthrene from medium by stimulating bacteria and C230 activities.