Effects of Crassocephalum bauchiense (Hutch) leaf aqueous extract on toxicity indicators and reproductive characteristics in Oryctolagus cuniculus exposed to potassium dichromate

摘要

Objective:To evaluate the effects of Crassocephalum(C.)bauchiense(Hutch)leaf aqueous extract on the biochemical markers of toxicity,oxidative stress indicators and reproductive parameters in rabbit does(Oryctolagus cuniculus)exposed to potassium dichromate.Methods:A total of 36 nulliparous and sexually mature female rabbit does,aged 8 months and weighing 2.8-3.0 kg,were divided into 6 groups of 6 animals.After mating,group T0 received distilled water(1 mL/kg body weight),while groups T0-,VC100,AE100,AE200 and AE400 were treated with 40 mg/mL/kg body weight of potassium dichromate.In addition,group VC100 received 100 mg/mL/kg body weight of vitamin C,while groups AE100,AE200 and AE400 received aqueous extract of C.bauchiense leaves at 100,200 and 400 mg/mL/kg body weight,by oral gavage for 27 days,respectively.On the 28th daypost-co?tum,animals were sacrificed.Biochemical indicators such as creatinine,urea,alanine amino tranferase,aspartate amino transferase,total protein and total cholesterol were measured by using a spectrophotometer.Follicle stimulating hormone,luteinizing hormone and progesterone were measured by enzyme-linked immuno sorbent assay.Oxidative stress markers like malondialdehyde,catalase,superoxide dismutase and total peroxidase in ovary homogenates were measured by spectrophotometer.Results:Serum concentrations of urea,creatinine,alanine aminotransferase,aspartate aminotransferase and total cholesterol were significantly higher(P0.05)among all groups;C.bauchiense leaf extracts significantly(P0.05).Catalase and total peroxidase activities significantly decreased(P<0.05),and malondialdehyde significantly increased in T0-group compared to other groups.The administration of C.bauchiense leaf extracts significantly(P<0.05)increased the activities of catalase and peroxidase.Conclusions:Potassium dichromate-induced oxidative stress involves in hepatotoxicity,nephrotoxicity and reprotoxicity.The aqueous extract of C.bauchiense leaves could mitigate these adverse effectsvia antioxidant properties.