Effect of hematopoietic stem cell on tumor necrosis factor-αexpression, spiral artery remodeling and placental apoptosis in lead-exposed pregnant mice

摘要

Objective:To evaluate the effectiveness of hematopoietic stem cell (HSC) on TNF-αexpression, spiral artery remodeling, and placental apoptosis.Methods: The study used pregnant mice divided to five groups of treatment: Group 1, without treatment;Group 2, 1/12 LD50 of lead and Aquadest;Group 3, 1/16 LD50 of lead and Aquadest, Group 4, 1/12 LD50 of lead and 1×107 HSC;and group 5, 1/16 LD50 of lead and 1×107 HSC. Histopathology examination was performed to identify spiral artery remodeling. Immunohistochemistry analysis was conducted to characterize TNF-α expression and the apoptosis index. The data from all five groups were compared using the ANOVA test.Results:TNF-α expression differed significantly among the groups that were not exposed to lead, exposed to lead and exposed to lead after HSC treatment (P<0.01). There was also a significant difference of spiral artery remodeling between groups without lead-exposed, lead exposed and lead exposed followed HSC treatment (P<0.05). In apoptosis index evaluation there was a significant difference between groups without lead-exposed, lead exposed and lead exposed followed HSC treatment, but not in the groups of 1/16 LD50 of lead followed HSC (P>0.05).Conclusions:In lead-exposed pregnant mice, the administration of hematopoietic stem cell may decrease TNF-α expression, spiral artery remodeling, and placental apoptosis index but in the group of 1/16 LD50 of lead and HSC treatment, apoptosis index has no differences with those of control group.