Shotgun lipidomic analysis of simple lipids in rapeseed oil by electrospray ionization tandem mass spectrometry

摘要

Rapeseed is widely grown in China as an important oil crop.In this study,Shotgun lipidomic methodology based on tandem mass spectrometry but without liquid chromatography was established for simple lipids analysis such as mono-,di-and triacylglycerols(MAGs,DAGs and TAGs)and the derivatives such as free fatty acids(FFAs).Six varieties of rapeseeds belonging to 3 types(B.juncea,B.campestris and B.oleracea)from different areas of China were investigated,with 59 TAG species,10 DAG species,5 MAG species and 6 FFA species identified.It was found that erucic acid was mainly present in the form of TAG 62:4(22:1/18:2/22:1),TAG 62:3(22:1/18:1/22:1),and TAG 62:5(22:1/18:3/22:1)in high erucic acid rapeseed oils,while oleic acid was mainly present in the form of TAG 54:2(18:0/18:1/18:1),and TAG 54:4(18:1/18:2/18:1)in high oleic acid rapeseed oils.It was difficult for DAG,MAG and FFA quantification due to their low content with high background content of TAGs.Results showed that the main differences in lipids were not derived from the types of rapeseed,but directly related to varieties.