Cellular antioxidant activity and cytotoxicity assay of canolol

摘要

To better understand canolol as an efficient intracellular antioxidant agent in food related fields,potential cellular antioxidant activity and cytotoxicity were evaluated using multiple assays,including cellular antioxidant activity(CAA),3-(4,5)-dimethylthiahiazo(-z-y1)-3,5-di-phenytetrazoliumromide(MTT)and methylene blue assay.CAA assay was used to identify the half maximal effective concentration of canolol and other bioactive components,such as sinapic acid,resveratrol and sesamol in oil crops.Results showed that antioxidant capacity(without PBS wash)varies between different compounds.Capacity variation followed the order:sinapic acid>canolol>resveratrol>sesamol.While with PBS wash,the order changed to sinapic acid>resveratrol>canolol>sesamol.Half maximal inhibitory concentration of canolol measured by MTT was 1887.08±1.22μmol/L,implying that cytotoxicity of canolol was greatly lower than that of resveratrol,sesamol and sinapic acid.Methylene blue assay showed that cell viability was still up to 90%exposed to canolol at a high concentration of 4444μmol/L.It suggested that canolol had desired antioxidant activity in organism with low cellular cytotoxicity.