中华哲水蚤(Calanus sinicus)个体核酸含量及其对生长状况的指示作用

摘要

Individual nucleic acids (RNA and DNA) concentrations of Calanus sinicus collected in May 2009 and November 2010 were measured by microplate fluorescent assay,and RNA ∶ DNA ratio as a growth indicator of C.sinicus was applied.Without food supply,individual RNA concentration and RNA ∶ DNA ratios were found declined significantly in 24 hours.Individual RNA concentration and RNA ∶ DNA ratio of male C.sinicus were both lower than female and C5,in which no significant difference in the DNA concentration was found among them.RNA concentration and RNA ∶ DNA ratios were different among stations and high values were found in nearshore stations.There was no significant difference in DNA concentration among stations and between the two surveys.Correlation analyses suggested that external food are essential for the growth of C.sinicus.%采用荧光染色法对黄、东海春、秋季中华哲水蚤(Calanus sinicus)个体核酸含量进行了研究,初步探讨了RNA∶DNA比对中华哲水蚤生长状况的指示作用.结果表明,中华哲水蚤在饥饿培养24h后RNA∶DNA比值显著降低;雄性个体RNA含量和RNA∶DNA比值显著低于雌体和C5期幼体；春、秋季(2009年5月和2010年11月)个体RNA含量和RNA∶DNA比值存在显著的空间变化,两者最高值均出现在近岸；两个月份DNA含量相对稳定,在2.5-3.5μg/ind之间；个体RNA含量和RNA∶DNA比值与叶绿素之间显著正相关,说明饵料是中华哲水蚤的生长过程中重要的影响因素.