The primary effective components of Xuefuzhuyu,including Rhizoma Chqanxiong,Radix Salviae Miltiorrhiae,and Radix Angelicae Sinensis,have been shown to induce the differentiation of bone marrow mesenchymal stem cells(BMMSCs) into neuronal-like cells.However,there is little available evidence on the effects of the Chinese herbal compound Xuefuzhuyu on BMMSC differentiation.The present study investigated the effects of Xuefuzhuyu on differentiation of rat BMMSCs into neuronal-like cells,and evaluated the optimal concentration for inducing differentiation.Inverted microscopy was used to observe BMMSCs induced by Xuefuzhuyu;immunocytochemistry revealed expression of the neuronal marker neuron-specific enolase surface antigen in the majority of BMMSCs following treatment with Xuefuzhuyu at concentrations of 1,3,5,and 10 g/L.A concentration of 3 g/L resulted in highest neuron-specific enolase expression.These results suggest that BMMSCs can be induced to differentiate into neuronal-like cells with Xuefuzhuyu,and 3 g/L is the optimal inductive concentration for effective differentiation.
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