Previous studies have demonstrated that differentiated neural stem cells(NSCs) are more suitable for transplantation than non-differentiated NSCs.In this study,NSCs were expanded in vitro for two passages,induced with retinoic acid to differentiate,and harvested between 1-6 days later.They were subsequently cultured in artificial cerebrospinal fluid for an additional 3 days,during which their growth and morphology was monitored.NSCs induced for 4 days exhibited a peak rate of cells differentiating into neurons and robust growth.Our results indicate that the optimal time point for transplanting NSCs is following a 4-day period of induced differentiation.
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