Effects of all-trans retinoic acid on metabolic gene expression in the glioma cell line SHG-44

摘要

BACKGROUND:Genetic abnormalities and changes in gene expression have been shown in various grades of glioma.However,the relationship between gene expression patterns and pathways related to malignant transformation of glioma remains poorly understood. OBJECTIVE:To screen differentially expressed genes between normal and all-trans retinoic acid-treated glioma cell line SHG-44 cells with a complementary DNA(cDNA) microarray. DESIGN,TIME AND SETTING:The genomics,in vitro study was performed at the Laboratory of Neurobiology,Third Military Medical University of Chinese PLA,China from January to October 2007. MATERIALS:The glioma cell line SHG-44 was provided by the Third Military Medical University of Chinese PLA.All-trans retinoic acid was purchased from Sigma,USA.cDNA microarray was purchased from City University of Hong Kong. METHODS:The glioma cell line SHG-44 was treated with 10μmol/L all-trans retinoic acid for 3 days. Differentiation-related genes were determined using cDNA microarray. MAIN OUTCOME MEASURES:Gene expression patterns were compared between normal and all-trans retinoic acid-treated SHG-44 cells.Differentially expressed genes were randomly selected and determined by Northern blot analysis. RESULTS:Northern blot analysis revealed downregulated RPL 13 gene expression and upregulated SOD2 gene expression,which was identical to cDNA microarray results.Five differentially expressed genes(TPI1,BPGM,ALDOA,LDHA,and RRM1) were shown to be involved in cell metabolism,in six metabolic pathways.Four differentially expressed genes(TPI1,BPGM,ALDOA, and LDHA) were associated with carbohydrate metabolism,such as fructose metabolism,pyruvic acid metabolism,pentose phosphate pathway,glycolysis,and gluconeogenesis.One differentially expressed gene(RRM1) was correlated with purine and pyrimidine metabolism. CONCLUSION:Five metabolic genes(TPI1,BPGM,ALDOA,LDHA,and RRM1),which participate in cell carbohydrate and nucleotide metabolism,were shown to closely correlate with glioma development.