石杉碱甲对阿尔茨海默病转基因小鼠脑内β-淀粉样蛋白表达的影响

摘要

本文主要观察石杉碱甲对阿尔茨海默病转基因小鼠脑内β-淀粉样蛋白表达的影响.应用免疫荧光技术检测Aβ在C57BL/6野生型小鼠、APP/PS1转基因小鼠及石杉碱甲治疗组小鼠脑内的表达情况,同时应用Western blot和Real-Time PCR的方法检测Aβ形成所需的β-、γ-分泌酶亚基Bace1及PS1在脑内的表达.结果发现石杉碱甲治疗后的AD模型小鼠脑内Aβ表达及含量与AD组相比明显减少,Bace1及PS1的表达都有明显的减少.以上结果说明石杉碱甲可通过降低APP/PS1转基因小鼠脑内Bace1、PS1的表达进而降低γ-分泌酶的活性,从而使AD小鼠脑内的Aβ蛋白表达水平下降,提示石杉碱甲在AD的临床治疗方面具有重要的理论意义.%In this study,the effect of huperzine A on the expression of amyloidβ-protein (Aβ) in the brain of transgenic mice was investigated.The levels of Aβ in wild type,APP/PS1 transgenic mice group and the treatment mice group were tested immunofluorescence.The expression of Bace1 and PS1 which are essential forβ-/γ-secretase activity was detected by Western blot and Real-Time PCR assays.It was found that the activity and content of Aβ in the huperzine A administration mice brain were decreased compared to that of the AD model mice,and the mRNA and protein expression levels of Bace1 and PS1were suppressed in the brain of huperzine A treatment mice group.These results prompted that huperzine A can decrease Aβ in AD mice brain through suppressing the activity of β-/γ-secretase in APP/PS1 transgenic mice brain.These results suggested that huperzine A had important theoretical significance in the clinical treatment of AD.