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The Arabidopsis LFR Gene Is Required for the Formation of Anther Cell Layers and Normal Expression of Key Regulatory Genes

机译:拟南芥LFR基因是花药细胞层形成和关键调控基因正常表达所必需的

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摘要

The anther is the male reproductive organ in flowering plants.Although some genes were reported to be involved in anther development,the molecular mechanisms underlying the transcriptional regulation of these genes is unclear.Ifr-2 (leaf and flower related-2),the null allele of Arabidopsis thaliana LFR (LEAF AND FLOWER RELATED),was male-sterile.The anthers of Ifr-2 plants were defective in sporogenous cell formation,tapetum development,and pollen development.In agreement with these phenotypes,expression studies showed that LFR was expressed in all cell layers of the anther,and that expression was particularly strong in the tapetal cells and pollen grains.Quantitative RT-PCR analysis revealed that LFR is required for the normal transcription of some anther development-related genes,such as AMS,CALSS,and DYT1,MS1 and MS2,and ROXY2.Genetic analysis showed that SPL was epistatic to LFR while LFR was epistatic to DYT1.We propose that LFR may be a crucial component in the regulation of a genetic network that modulates anther development.
机译:花药是开花植物中的雄性生殖器官。尽管据报道某些基因参与花药的发育,但这些基因转录调控的分子机制尚不清楚。Ifr-2(叶和花相关2),无效拟南芥LFR(叶和花相关)的等位基因是雄性不育的。Ifr-2植物的花药在孢子形成,花序发育和花粉发育方面存在缺陷。与这些表型一致,表达研究表明LFR是RT-PCR定量分析显示,LFR是某些与花药发育相关的基因,如AMS,CALSS的正常转录所必需的,在花药的所有细胞层中都有表达,并且在绒毛细胞和花粉粒中的表达特别强。遗传分析表明,SPL对LFR具有上位性,而LFR对DYT1具有上位性。我们认为LFR可能是基因调控的重要组成部分。调节花药发育的网络。

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  • 来源
    《分子植物(英文版)》 |2012年第5期|993-1000|共8页
  • 作者单位

    Key Laboratory of Molecular Cell Biology in Hebei Province, College of Life Science, Hebei Normal University, Shijiazhuang 050016, China;

    Key Laboratory of Molecular Cell Biology in Hebei Province, College of Life Science, Hebei Normal University, Shijiazhuang 050016, China;

    Key Laboratory of Molecular Cell Biology in Hebei Province, College of Life Science, Hebei Normal University, Shijiazhuang 050016, China;

    Key Laboratory of Molecular Cell Biology in Hebei Province, College of Life Science, Hebei Normal University, Shijiazhuang 050016, China;

  • 收录信息 中国科学引文数据库(CSCD);中国科技论文与引文数据库(CSTPCD);
  • 原文格式 PDF
  • 正文语种 chi
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