Dear Editor, Targeted genome-editing tools are very important and efficient for plant functional genomics research, especially when proper mutants are not available.CRISPR/Cas9-based genome editing is a recently developed powerful technology derived from the bacterial clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) defense system, whereby CRISPR loci were transcribed into non-coding RNAs, which can form a complex with Cas proteins to mediate the cleavage of complementary invading DNA.
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