Dear Editor, Eliminating misfolded or mistargeted proteins is crucial for cell viability because these proteins accumulate as non-specific aggregates,which can be toxic to the cell (Lee et al.,2009;Sroka et al.,2009).Previously,we have shown that in ppi2 (plastid protein import 2) mutant plants,the transcript levels of Hsc70-4 (one isoform of the Hsc70 family) and CHIP (an E3 ligase) were highly upregulated,which ultimately plays crucial roles in proteasomal degradation of unimported plastid proteins (Lee et al.,2009).We also found that,along with those of Hsc70-4 and CHIP,the transcript level of AtBAG1 (Arabidopsis thaliana Bcl2-associated athanogene 1) in the ppi2 mutant was 2.38-fold higher than that in the wild-type (Lee et al.,2009).In mammalian cells,BAG proteins play multiple roles in protein homeostasis,especially as nucleotide exchange factors of Hsc70,thereby contributing to protein quality control (Alberti et al.,2003;Doukhanina et al.,2006).Therefore,in this study,we examined the role of AtBAG in Hsc70-4-mediated protein quality control in the cytosol.
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