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首页> 外文期刊>分子植物（英文版） >The Intronic cis Element SE1 Recruits trans-Acting Repressor Complexes to Repress the Expression of ELONGATED UPPERMOST INTERNODE1 in Rice
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The Intronic cis Element SE1 Recruits trans-Acting Repressor Complexes to Repress the Expression of ELONGATED UPPERMOST INTERNODE1 in Rice

机译：内含子顺式元件SE1招募反式阻遏物复合物，以抑制水稻中ELONGATED UPPERMOST INTERNODE1的表达

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摘要

Plant height has a major effect on grain yield in crops such as rice (Oryza sativa),and the hormone gibberellic acid (GA) regulates many developmental processes that feed into plant height.Rice ELONGATED UPPERMOST INTERNODE1 (Eui1) encodes a GA-deactivating enzyme governing elongation of the uppermost internode.The expression of Eui1 is finely tuned,thereby maintaining homeostasis of endogenous bioactive GA and producing plants of normal plant height.Here,we identified a dominant dwarf mutant,dEui1,caused by the deletion of an RY motif-containing cis-silencing element (SE1) in the intron of Eui1.Detailed genetic and molecular analysis of SE1 revealed that this intronic cis element recruits at least one trans-acting repressor complex,containing the B3 repressors OsVAL2 and OsGD1,the SAP18 corepressor,and the histone deacetylase OsHDA710,to negatively regulate the expression of Eui1.This complex generates closed chromatin at Eui1,suppressing Eui1 expression and modulating GA homeostasis.Loss of SE1 or dysfunction of the complex components impairs histone deacetylation and H3K27me3 methylation of Eui1 chromatin,thereby increasing Eui1 transcription and decreasing bioactive GA,producing dwarfism in rice.Together,our results reveal a novel silencing mechanism in which the intronic cis element SE1 negatively regulates Eui1 expression via repressor complexes that modulate histone deacetylation and/or methylation.
机译：株高对水稻（Oryza sativa）等农作物的籽粒产量具有重要影响，而赤霉素（GA）调节着进入株高的许多发育过程。水稻长粒高INTERNODE1（Eui1）编码一种使GA失活的酶控制Eui1的表达，从而维持内源性生物活性GA的稳态并产生正常株高的植物。在这里，我们鉴定出一个主要的矮化突变体dEui1，该突变体是由RY基序的缺失引起的。在Eui1的内含子中含有顺式沉默元件（SE1）。对SE1的详细遗传和分子分析表明，该内含子顺式元件募集了至少一种反式作用阻遏物复合物，其中包含B3阻遏物OsVAL2和OsGD1，SAP18核心阻遏物以及组蛋白脱乙酰基酶OsHDA710负调控Eui1的表达。该复合物在Eui1处产生封闭的染色质，抑制Eui1的表达并调节GA稳态。 SE1的丧失或复杂成分的功能障碍会损害Eui1染色质的组蛋白脱乙酰基和H3K27me3甲基化，从而增加Eui1转录并降低生物活性GA，从而使水稻产生矮化。我们的结果共同揭示了一种新型的沉默机制，其中内含子顺式元素SE1为负通过调节组蛋白去乙酰化和/或甲基化的阻遏物复合物调节Eui1表达。

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  • 来源
    《分子植物（英文版）》 |2018年第5期|720-735|共16页
  • 作者

    Yongyao Xie; Yuanling Chen; Yoji Kawano; Yao-Guang Liu; Letian Chen; Yaling Zhang; Jingluan Han; Jikai Luo; Gousi Li; Jianle Huang; Haibin Wu; Qingwei Tian; Qinlong Zhu;

  • 作者单位

    State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou 510642, China;

    Guangdong Provincial Key Laboratory of Protein Function and Regulation in Agricultural Organisms, South China Agricultural University, Guangzhou 510642, China;

    Key Laboratory of Plant Functional Genomics and Biotechnology of Guangdong Provincial Higher Education Institutions, South China Agricultural University,Guangzhou 510642, China;

    College of Life Sciences, South China Agricultural University, Guangzhou 510642, China;

    State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou 510642, China;

    Key Laboratory of Plant Functional Genomics and Biotechnology of Guangdong Provincial Higher Education Institutions, South China Agricultural University,Guangzhou 510642, China;

    College of Life Sciences, South China Agricultural University, Guangzhou 510642, China;

    Shanghai Center for Plant Stress Biology, Chinese Academy of Sciences, Shanghai 201602, China;

    State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou 510642, China;

    Key Laboratory of Plant Functional Genomics and Biotechnology of Guangdong Provincial Higher Education Institutions, South China Agricultural University,Guangzhou 510642, China;

    College of Life Sciences, South China Agricultural University, Guangzhou 510642, China;

    State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou 510642, China;

    Guangdong Provincial Key Laboratory of Protein Function and Regulation in Agricultural Organisms, South China Agricultural University, Guangzhou 510642, China;

    Key Laboratory of Plant Functional Genomics and Biotechnology of Guangdong Provincial Higher Education Institutions, South China Agricultural University,Guangzhou 510642, China;

    College of Life Sciences, South China Agricultural University, Guangzhou 510642, China;

    State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou 510642, China;

    Guangdong Provincial Key Laboratory of Protein Function and Regulation in Agricultural Organisms, South China Agricultural University, Guangzhou 510642, China;

    Key Laboratory of Plant Functional Genomics and Biotechnology of Guangdong Provincial Higher Education Institutions, South China Agricultural University,Guangzhou 510642, China;

    College of Life Sciences, South China Agricultural University, Guangzhou 510642, China;

    State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou 510642, China;

    Guangdong Provincial Key Laboratory of Protein Function and Regulation in Agricultural Organisms, South China Agricultural University, Guangzhou 510642, China;

    Key Laboratory of Plant Functional Genomics and Biotechnology of Guangdong Provincial Higher Education Institutions, South China Agricultural University,Guangzhou 510642, China;

    College of Life Sciences, South China Agricultural University, Guangzhou 510642, China;

    State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou 510642, China;

    College of Life Sciences, South China Agricultural University, Guangzhou 510642, China;

    State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou 510642, China;

    College of Life Sciences, South China Agricultural University, Guangzhou 510642, China;

    State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou 510642, China;

    College of Life Sciences, South China Agricultural University, Guangzhou 510642, China;

    College of Life Sciences, South China Agricultural University, Guangzhou 510642, China;

    State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou 510642, China;

    College of Life Sciences, South China Agricultural University, Guangzhou 510642, China;

    State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou 510642, China;

    Key Laboratory of Plant Functional Genomics and Biotechnology of Guangdong Provincial Higher Education Institutions, South China Agricultural University,Guangzhou 510642, China;

    College of Life Sciences, South China Agricultural University, Guangzhou 510642, China;

  • 收录信息 中国科学引文数据库(CSCD);中国科技论文与引文数据库(CSTPCD);
  • 原文格式 PDF
  • 正文语种 eng
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