野茉莉叶水提物抑菌、抗炎及止咳作用的实验研究

摘要

目的:探讨野茉莉叶水提物抑菌、抗炎及止咳作用,验证其相关功效,为该药的临床应用提供参考依据.方法:通过体外抑菌实验观察其抑菌作用,通过大鼠棉球植入实验、足趾肿胀实验和小鼠耳廓肿胀实验等评价其抗炎作用,通过小鼠氨水引咳实验评价其镇咳作用.结果:野茉莉叶水提物可抑制大肠埃希氏菌、肺炎克雷伯氏菌、沙门氏菌及金黄色葡萄球菌的生长,但对白色念珠菌的生长无抑制作用;野茉莉叶水提物高剂量明显降低大鼠棉球植入肉芽肿净重;低、高剂量均明显甚至显著抑制角叉菜胶引起的大鼠足趾肿胀,并能明显甚至显著改善二甲苯涂布引起的耳廓肿胀;中、高剂量均显著延长氨水引发的咳嗽潜伏期,低、中、高剂量组均显著减少氨水引发的咳嗽次数.结论:本实验条件下,野茉莉叶水提物具有抑菌、抗炎及止咳的功能,与其相关的中医临床功效及主治相符,为其临床应用提供了一定的实验依据.%This study was aimed to explore the in vitro antibacterial,anti-inflammatory and antitussive effects of water extract of Styrax japonicus leaves and to verify its concerning efficacies.In vitro antibacterial,anti-inflammatory and antitussive effects were observed by in vitro antibacterial experiment,cotton ball implantation,carrageenan-induced paw edema together with xylene-induced ear edema experiments with rats or mice,and ammonia hydroxide-induced coughing experiment,respectively.The results showed that water extract of Styraxjaponicus leaves can inhibit the growth of Escherichia coli,Klebsiella pneumoniae,Salmonella and Staphylococcus aureus except candida albicans.Its high-dose group can reduce the weight of cotton ball granuloma in rats obviously.And its low-dose and high-dose groups can inhibit carrageenan-induced paw edema apparently.Meanwhile,its low-dose and high-dose groups can improve xylene-induced ear edema distinctively.Its low-dose and high-dose groups can evidently prolong the ammonia hydroxide-induced coughing incubation periods.And all groups can reduce coughing times.It was concluded that the water extract of Styrax japonicus leaves coincided with the relative clinical efficacy of traditional Chinese medicine (TCM) and provided experimental evidences for its clinical application to some degree.