青蒿素对糖尿病大鼠肾组织 AP -1的 DNA结合活性升高的抑制作用

摘要

目的：探讨青蒿素对实验糖尿病大鼠肾组织转录因子活化蛋白-1（AP-1）的DNA结合活性升高的抑制作用。方法将75只实验大鼠分成正常对照组（ A组）、糖尿病非治疗组（B组）及青蒿素治疗组（C组），B、C组应用腹腔单剂量注射链脲佐菌素（STZ）65 mg/kg建立糖尿病大鼠模型。 C组给予腹腔注射青蒿素300 mg/（ kg· d）。于实验进行到第4周时处死各组大鼠，取部分右肾皮质，应用电泳迁移率改变分析（ EMSA）法观察青蒿素对实验糖尿病大鼠肾组织AP-1的DNA结合活性的影响，并制备病理切片以观察肾组织的病理学变化。结果 B组大鼠肾细胞 AP-1的DNA结合活性较A组显著上升，C组大鼠对肾细胞AP-1的DNA结合活性有显著抑制作用，并显著改善糖尿病大鼠肾脏组织的病理变化。结论青蒿素通过下调糖尿病大鼠肾组织AP-1的DNA结合活性上调而改善糖尿病大鼠肾脏组织病理改变。%Objective It is to approach the inhibitory effect of artemisinin on the upregulation of the DNA -binding activi-ty of AP-1 in the kidney tissue of experimental diabetic rats .Methods 15 rats were divided into normal control rats ( group A), STZ-induced diabetic rats (group B), STZ-induced diabetic rats treated with artemisinin 300 mg/(kg· d) intraper-itoneally (group C).The DNA-binding activity of AP-1 in the kidney tissue was detected by electrophoretic mobility shift assay ( EMSA) , while renal pathologic changes were observed after 4 weeks of treatment .Results The DNA-binding activity of AP-1 was significantly activated in renal cortices in untreated -diabetic rats after 4 weeks.The abnormal level of the DNA-binding activity of AP-1 was partly reversed in diabetic rats treated by artemisinin .Accordingly , renal pathologic changes in diabetic rats treated with artemisinin were significantly improved .Con clusion Artemisinin has some renoprotective effects in experimental diabetic rats partly through inhibiting the DNA -binding activity of AP-1 in renal cortices .