蓬草除痹汤对类风湿关节炎免疫调节作用的实验研究

摘要

目的：观察蓬草除痹汤（ PCCB）对Ⅱ型胶原诱导关节炎（ CIA）大鼠模型外周血中细胞因子IL-17、IL-1β、TNF-α及T细胞亚群CD4＋T、CD8＋T、CD4＋T/CD8＋T的干预作用，探讨PCCB治疗类风湿关节炎（ RA）的抗炎免疫作用机制。方法建立CIA大鼠模型，随机分为PCCB组、雷公藤组、空白组及模型组，连续给药28 d，采用酶联免疫吸附（ ELISA）法、流式细胞术评价PCCB对CIA大鼠关节炎指数（AI）及大鼠血清IL-17、IL-1β、TNF-α和CD4＋T、CD8＋T、CD4＋T/CD8＋T表达的影响。结果治疗28 d后，PCCB组、雷公藤组关节肿胀度及AI显著改善（P均＜0．01）；血清IL-17、IL-1β、TNF-α水平明显降低（P均＜0．01）；CD4＋T降低，CD8＋T升高，CD4＋T/CD8＋T显著降低，且PCCB组的抑制作用明显优于雷公藤组（P＜0．05）。结论 PCCB能有效改善CIA大鼠关节炎指数，下调大鼠血清中致炎因子IL-17、IL-1β、TNF-α的水平，调节大鼠血清中CD4＋T、CD8＋T及CD4＋T/CD8＋T 比例，这可能是PCCB治疗RA的主要机制，为临床应用及进一步研究奠定了基础。%Objective It is to observe the intervention effect on cytokine of IL -17 , IL-1β, TNF-αand T-lymphocyte subset of CD4 +T,CD8 +T and CD4 +T/CD8 +T in peripheral blood of rats with collagen -induced arthritis Ⅱ( CIA) treated by the Peng Cao rheumatism -syndrome eliminating decoction ( PCCB, abbreviation for the decoction's Chinese name: Peng Cao Chu Bi ) , and to explore its mechanism of immune anti -inflammatory action .Methods CIA rat models were established and randomly divided them into 4 groups:PCCB group , tripterygium glycosides group , blank group and model group , and all the groups were treated for 28 days.Enzyme-linked immunosorbent assay (ELISA) and flow cytometry were used to evaluate the effects on arthritis index ( AI) and on the expression levels of IL -17 , IL-1βand TNF-αand T-lymphocyte subset of CD4 +T,CD8 +T and CD4 +T/CD8 +T in serum of CIA rats treated by PCCB .Results After 28 days'treatment, compared with model group, the AI of the rats in the PCCB and TG groups were significantly improved (P<0.01), the levels of IL-17, IL-1βand TNF-αin serum were reduced (P<0.01), CD4 +were reduced, CD8 +increased (P<0.01) and CD4 +T/CD8 +T reduced obviously (P<0.05), while the inhibiting effect in PCCB group were superior obviously to that in TG group (P<0. 05).Conclusion PCCB could effectively improve the AI of CIA rats , reduce the level of pro-inflammatory cytokine of IL -1, IL-1βand in serum, regulate the ratio of CD4 +T,CD8 +T and CD4 +T/CD8 +T in serum of the rats.That might suggest a key mechanism to remedy RA by PCCB , which may lay a foundation of clinical application for RA treatment .