CD68＋、CD163＋巨噬细胞对食管癌浸润及预后的影响

摘要

Objective It is to approach the influence of CD68+, CD163+ macrophage on the infiltration of esophageal cancer and its prognosis .Methods 84 cases of specimens of esophageal carcinoma in resection operation and 49 cases of e-sophageal paracancerous tissues were collected , the infiltration density of CD68+, CD163+ macrophage in the specimens were detected by immunohistochemistry method , and the prognosis of the patients were analyzed .Results The every field mean den-sity of CD68 +, CD163 + macrophages infiltrating in esophageal cancer tissue was 35.69 ±14.50 and 29.78 ±12.36 respec-tively, the median was 32.8 and 27.1.The results in paracancerous tissues was 4.26 ±2.30 and 2.33 ±1.71, the median was 3.4 and 2.2 respectively, the median was 32.8 and 27.1.The infiltration density of CD68+ and CD163 +macrophage in esophageal cancer tissue was significantly higher than that in paracancerous tissues (all P <0.05).The infiltration density of CD68+, CD163+ macrophage with TNM III stages, lymph node metastasis, or low differentiation degree, was significantly higher than that with TNM stage I to II , without lymph node metastasis , and high differentiation degree (all P <0.05).The survival rates after operation of 1 year, 3 years, 5 years were 83%, 67% and 46%, the median survival time was 44.21 months.Spearman correlation analysis showed that there was negative correlation between the infiltration density of CD 68+, CD163 + macrophage and the survival time of patients (P <0.05); multivariate Cox regression analysis showed that the TNM stage, lymph node metastasis, differentiation, CD163+ macrophage infiltration density affects the prognosis of patients with in -dependent risk factors ( all P <0.05).Conclusion The CD68+, CD163+ macrophage infiltration density in local lesion tis -sue of patients with esophageal cancer are increased significantly , the? increase of CD163+ macrophages infiltration density is an independent factor to influence the prognostic .%目的：探讨 CD68＋、CD163＋巨噬细胞对食管癌浸润及预后的影响。方法收集手术切除的食管癌组织标本84例、食管癌旁组织标本49例，采用免疫组化法检测组织标本中 CD68＋、CD163＋巨噬细胞浸润密度，并对患者预后进行分析。结果食管癌组织中CD68＋、CD163＋巨噬细胞浸润密度每视野（35．69±14．50）个和（29．78±12．36）个，中位数分别为32．8个和27．1个；癌旁正常组织中 CD68＋、CD163＋巨噬细胞浸润密度每视野（4．26±2．30）个和（2．33±1．71）个，中位数分别为3．4个和2．2个。食管癌组织中 CD68＋、CD163＋巨噬细胞浸润密度明显高于癌旁正常组织（P 均＜0．05）。 TNMⅢ期者、有淋巴结转移者、低分化程度者 CD68＋、CD163＋巨噬细胞浸润密度明显高于 TNM Ⅰ～Ⅱ期者、无淋巴结转移者、高中分化程度者（P 均＜0．05）；患者术后1年、3年、5年生存率分别为83％，67％，46％，中位生存时间为44．21个月。Spearman 相关分析显示，CD68＋、CD163＋巨噬细胞浸润密度与患者生存时间呈现负相关性（P ＜0．05）；多因素 Cox 回归分析 TNM 分期、淋巴结转移、分化程度、CD163＋巨噬细胞浸润密度是影响患者预后的独立危险因素（P 均＜0．05）。结论食管癌患者局部病灶组织中 CD68＋、CD163＋巨噬细胞浸润密度明显增高，CD163＋巨噬细胞浸润密度增高是影响患者预后的独立因素。