正心力颗粒对睡眠剥夺导致的认知能力和应激适应能力下降的改善作用

摘要

目的 对中药复方正心力颗粒改善机体应激适应能力的效能进行初步临床观察.方法 以72 h睡眠剥夺为应激源,采用放射免疫法测定机体血浆皮质醇水平,免疫印迹法测定外周血淋巴细胞热休克蛋白70(Hsp70)水平,并分别以视觉反应时、记忆广度及闪光融合频率反映机体的反应能力、短时记忆能力和大脑皮质疲劳状况.结果 睡眠剥夺后,机体血浆皮质醇水平升高(23.8±4.5)%,外周血淋巴细胞Hsp70表达增强,记忆能力和应激反应能力明显下降;正心力颗粒可抑制应激所致皮质醇的过量释放,诱导Hsp70的激活,显著增强应激机体的记忆能力和应激反应能力,纠正大脑皮质疲劳的发生;且安全无毒.结论 中药正心力颗粒具有较强的应激损伤防护功能,并具有明显提高机体应激适应能力和脑体作业能力的作用.%Objective To observe the clinical effect of Zhengxinli granules, a Chinese herbal compound, on adaptability of normal subjects to stress. Methods A 72 hour-sleep deprivation model was established. The levels of glucocorticoid in plasma and heat shock protein( Hsp70 ) in lymphocytes of peripheral blood were determined by radioimmunoassay and Western blotting. The time of visual response, memory span and flicker fusion frequency were used to reflect the faculty of reaction, short-term memory and the status of cerebral cortical defatigation, respectively. Results Zhengxinli granules, a Chinese herbal compound,effectively inhibited abnormal glucocorticoid secretion, induced protective Hsp70 expression and improved the cognitive ability of stressed subjects. Conclusion Zhengxinli granules,a Chinese herbal compound, function as a valid and safe traditional Chinese medicine for improving the adaptability of stress-injured subjects.