[Objective] The research aimed to discuss extraction of the chlorophyll a from phytoplankton by hot-ethanol method. [Method] The measurement method of chlorophyll a was improved. By contrast test,the best extraction temperature and time of chlorophyll a by hot-ethanol method were studied. Meanwhile,we contrasted hot-ethanol method with specification. [Result]It was simple to use hot-ethanol method to extract chlorophyll a from phytoplankton,and it was safe and reliable. When extraction temperature was 75 ℃,extraction efficiency of the chlorophyll a was the highest using hot ethanol to hot for 3 min. Chlorophyll a extracted by specification presented significant positive correlation with that by hot-ethanol method. Extraction efficiency of the hot-ethanol method was higher than that of the specification. [Conclusion]The research could provide theoretical reference for extraction of the chlorophyll a from phytoplankton in surface water.
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