Establishment of a Method for the Determination of Emodin in Wudajiangjun Liquor

摘要

[Objectives]To establish a method for the determination of emodin in Wudajiangjun liquor,a hospital preparation.[Methods]A high-performance liquid chromatography(HPLC)method for the determination of emodin in Rhizoma Polygontum Cuspidatum in hospital preparation Wudajiangjun liquor was established.Using HPLC method,octadecylsilane chemically bonded silica gel was used as filler for the chromatographic column[Inertsil-C 18 chromatographic column(5μm,4.6 mm×250 mm)].Methanol-0.1%phosphoric acid water(76∶24)was used as mobile phase.The flow rate was 1.0 mL/min,the column temperature was 30°C,the detection wavelength was 254 nm,and the injection volume was 10μL.[Results]The content of emodin in Wudajiangjun liquor was determined by reversed-phase high performance liquid chromatography(RP-HPLC).When the injection volume of emodin was in the range of 5.45-54.5μg/mL,there was a good linear relationship between the injection volume and the peak area.The regression equation is Y=42.952-15.068(r=0.9998),the average recovery rate is 98.23%,and RSD=1.45%.[Conclusions]A method for the determination of emodin in Wudajiangjun liquor by HPLC was established.This method can be used as a quality control method for Wudajiangjun liquor with high accuracy and good repeatability,which lays a foundation for the quality control of the medicinal liquor.