Effect of Ethanolic Extract of Pereskia aculeata Miller on Joint Swelling in Rats with Adjuvant-induced Arthritis

摘要

[Objectives]To explore the effect of ethanolic extract of Pereskia aculeata Miller(EEPA)on joint swelling in adjuvant arthritis rats.[Methods]60 SD rats were randomly selected.Except for the blank group,the remaining animals were injected with complete Freund''s adjuvant to establish the adjuvant arthritis rat models,which were divided into 6 groups:blank group,positive group,model group,and EEPA high-dose(64 mg/kg),EEPA medium-dose(32 mg/kg),EEPA low-dose(16 mg/kg)groups,10 in each group.The changes of ankle joint swelling diameter and arthritis index score were observed,and the spleen index was calculated.The serum prostaglandin E2(PGE2)level was detected by enzyme-linked immunosorbent assay(ELISA).[Results]The degree of ankle swelling diameter,arthritis index score,expression of serum inflammatory factors,and spleen index in the model group were significantly higher than those in the blank group,indicating that there was an inflammatory reaction and abnormal immune organs,and the modeling was successful.Under the intervention of EEPA drugs,the degree of ankle swelling diameter and arthritis index score of EEPA group with different doses were significantly lower than that of the model group(P<0.05),and the level of serum inflammatory factors was also lower than that of the model group(P<0.05).The index was lower than the model group and close to the normal group(P<0.05),and the higher the EEPA dose,the lower the index expression level(P<0.05).[Conclusions]EEPA can reduce joint swelling and inhibit the level of inflammatory factors to some extent in adjuvant arthritis rats.