Effects of Biological Fertilizer and Paclobutrazol on Total Flavonoids in Ophiopogon japonicus

摘要

[Objectives]The research aimed to study the effects of applying biofertilizer instead of paclobutrazol on the content of flavonoids in Ophiopogon japonicas and determine its quality characteristics,in order to provide the scientific basis for the quality control of GAP and the comprehensive utilization of resources.[Methods]O.japonicas collected in Lingxing Town and Huayuan Town of Sichuan Province was divided into three groups:blank control group,paclobutrazol group and biofertilizer group.Root tuber of O.japonicas was obtained,and the enzyme was killed by the microwave,and then it was dried in 60℃.The content of flavonoids in root tuber of O.japonicus was determined by ultraviolet spectrophotometry.Then,SPSS19.0 was used to analyze the results.[Results]The average contents of flavonoids in different treatments were as follows:0.184%-0.198% in control group;0.283%-0.330% in the paclobutrazol group;0.404%-0.428% in the biofertilizer group.Biological bacterial fertilizer had a significant role in promoting the synthetic accumulation of homoisoflavonoids in O.japonicus(P<0.001^**).[Conclusions]The application of biological fertilizer can not only significantly improve the content of homoisoflavonoids in root ruber of O.japonicus,but also significantly optimize the micro ecological environment of soil.Therefore,biofertilizer should be widely used to gradually replace paclobutrazol in the main production areas of O.japonicas.