Optimization of Ultrasonic Extraction Process of Oenanthe benghalensis Polysaccharide by Orthogonal Test

摘要

[Objectives]The research aimed to explore extraction method of Oenanthe benghalensis polysaccharide,and determine the optimal process of its ultrasonic extraction by orthogonal test.[Methods]Phenol-sulfuric acid method was used to determine polysaccharide s content.Based on single-factor experiment and orthogonal test,the influences of solid-liquid ratio(A),ultrasonic extraction temperature(B),ultrasonic power(C)and ultrasonic time(D)on extraction rate of O.benghalensis polysaccharide were studied,and the optimal process of ultrasonic extraction was selected.[Results]The influence order of solid-liquid ratio(A),ultrasonic extraction temperature(B),ultrasonic power(C)and ultrasonic time(D)on extraction rate of O.benghalensis polysaccharide was A >D >B >C.Via the orthogonal test,it was determined that the optimal extraction condition of O.benghalensis polysaccharide was as below:solid-liquid ratio 1∶25(g/mL),extraction temperature 60℃,extraction power 80 W,extraction time 40 min.Under the condition,extraction rate of O.benghalensis polysaccharide was 9.80%.[Conclusions]The process could provide the reference basis for extracting and producing O.benghalensis polysaccharides.