Effects of Huanglian Jiedu Decoction on Rat Gingivitis Induced by LPS

摘要

[Objectives]To evaluate the effects of Huanglian Jiedu Decoration(HLJD)on the treatment of rat gingivitis with excess internal heat through injecting lipopolysaccharide(LPS)in tooth-supporting tissues.[Methods]Gingival inflammation was established by LPS injection.Male SD rats were randomly divided into 7 groups:normal control group(no treatment group),solvent control group,model group(LPS injection),HLJD low dose group(0.25 g/kg),HLJD medium dose group(0.50 g/kg),HLJD high dose group(1.00 g/kg),and metronidazole and fenbufen capsule(MFC)group.Water intake and urine volume were observed through metabolic cage feeding;gingival tissue protein expressions were detected by LC-ESI-MS/MS method;oxidative stress-related molecules such as total superoxide dismutase(T-SOD)and hydrogen peroxidase catalase(CAT)were detected by the biochemical kit combined with a microplate reader;expressions of TSC related with energy metabolism-related signaling pathway were detected by western blotting.[Results]When rat gingivitis was injected by LPS,both water intake and urine volume were decreased,the levels of T-SOD and CAT were decreased,and the expressions of fructose-bisphosphate aldolase C,triosephosphate isomerase 1 and TSC2 were increased.Huanglian Jiedu Decoration can increase water intake and urine volume,increase the levels of T-SOD and CAT,reduce the expressions of fructose-bisphosphate aldolase C and triosephosphate isomerase 1,and inhibit the expression of energy metabolism-related pathway molecule TSC2.[Conclusions]Huanglian Jiedu Decoration can treat rat gingivitis through inhibiting energy metabolism and oxidative stress such as TSC signal pathway and glycolysis related molecules.