Determination of Oleanolic Acid in Achyranthes aspera L.of Different Production Areas by HPLC

摘要

[Objectives]To determine the content of oleanolic acid in 10 batches of Achyranthes aspera L.from different production areas,and to establish a high performance liquid chromatography(HPLC)method for the determination of oleanolic acid in A.aspera.[Methods]Agilent C 18 liquid chromatography column(250 mm×4.6 mm,5 μm)was used for gradient elution with acetonitrile∶water=61∶39 as the mobile phase.The flow rate was 1 mL/min,the detection wavelength was 210 nm,and the column temperature was 35℃.[Results]The oleanolic acid injection volume showed a good linear relationship with the peak area in the range of 0.382-7.640 μg.The linear equation of oleanolic acid was:A=530.76C,R=1.000 0;the range of sample recovery rate was 96.03%-102.73%,and the RSD value was 2.16%(n=9).The content of the oleanolic acid was the highest in A.aspera produced in Guilin City of Guangxi(0.92%),and the lowest oleanolic acid content was in sample of Sitang in Nanning City of Guangxi(0.27%).It is recommended that the oleanolic acid content of A.aspera should not be lower than 0.20%.[Conclusions]The HPLC method is accurate,reliable,easy to operate,and has good resolution.It is suitable for the determination of the content of oleic acid in A.aspera.It can provide reference for the quality control and standard drafting of A.aspera.