Content Determination of Quercetin in Ferment of Ginkgo biloba L.Leaves by HPLC

摘要

[Objectives]This study aimed to determine the content of quercetin in ferment of Ginkgo biloba L.leaves.[Methods]Bacillus licheniformis was selected for solid-state fermentation of G.biloba leaf powder,and the content of quercetin in ferment of G.biloba leaves was determined by reversed-phase high-performance liquid chromatography.First,the flavonoid glycosides were extracted with methanol.Then,the flavonoid glycosides were hydrolyzed with hydrochloric acid to prepare the test solution.The chromatographic conditions were as follows:Platisil ODS C 18 column(150 mm×4.6 mm,5μm);V methonal∶V water(0.4%phosphoric acid solution)=55∶45;flow rate of 1 mL/min;Shimadzu UV detector;detection wavelength of 360 nm.[Results]Quercetin was used as a reference substance.In the range of 0.002 6-0.036 0 g/L,there was a good linear relationship,with correlation coefficient of 0.999 8 and RSD of 1.26%.[Conclusions]This method is simple,easy to operate,accurate,and reproducible.It is suitable for the determination of quercetin content in G.biloba leaves.