Study on effects of Phyllanthusurinaria L extract Intervene Uric Acid in hyperuricemia mice

摘要

Objective:To investigate the effect ofPhyllanthusurinaria L extract on hyperuricemia mice.Methods:We were randomly divided into 6 groups by weight,control and model group,(15、30、45 mg/kg)ofPhyllanthusurinaria L extract group and allopurinol group.There were given oteracil potassium 300 mg/kg intragastrically to induce hyperuricemiamodel without control group,Phyllanthusurinaria L extract were given by intragastric administration,there were given saline solution instead as control and model group.Uric acid,blood urea ni-trogen and creatinine was detected in serum in n hyperuricemia mice for intragastric administration eight days.the contents and activity of xanthine oxidasein liver tissue were measured in hyperuricemia mice.The pathological changes of renal tissues were observed with HE staining in groups of mice.Western blot was surveied the expression of URAT1 protein in mice kidney tissues.Results:Groups ofPhyllanthusurinaria L extract could markedly reduce the uric acid,blood urea ni-trogen and creatinine level in serum on hyperuricemia mice,at the same time it reduce the contents and activity of liver in hyperuricemia mice,high dose group of Phyllanthusurinaria L extract did particularly well,close to allopurinol group,it could improve the pathologicalchanges in kidney tissueon hyperuricemia mice better than allopurinolgroup.the expressions of URAT1 protein of the model group was increased observbly than control group(P<0.05),but high dose group ofPhyllanthusurinaria L extract group wasdecreased more than model group,same level as control group(P<0.05).Conclusion:Phyllanthusurinaria L extractcould effectively were reduced the level of Uric acid in hyperuricemia mice,there were possibly reducing XOD activity,meanwhile,there were restrained the protein expression of URAT1 and decreased morphological changes in hyperuricemia mice.