Correlation of Wnt/β-catenin pathway changes with cytokines and apoptosis genes in diabetic foot wound

摘要

Objective:To study the correlation of Wnt/β-catenin pathway changes with cytokines and apoptosis genes in diabetic foot wound.Methods:diabetic foot patients admitted to our hospital between February 2015 and October 2017 were selected as the diabetic foot group,and non-diabetic patients admitted to our hospital during the same period were selected as the control group.Wound tissues of the diabetic foot group and normal skin tissues of the control group were collected to determine the mRNA expression levels of Wnt/β-catenin pathway molecules and apoptosis genes as well as the contents of cytokines.Results:The mRNA expression levels of Wnt1,β-catenin and B-cell lymphoma-2(Bcl-2)as well as the contents of vascular endothelial growth factor(VEGF),insulin-like growth factor-1(IGF-1)and basic fibroblast growth factor(bFGF)in the wounds of the diabetic foot group were significantly lower than those of the control group whereas the mRNA expression levels of glycogen synthase kinase-3(GSK-3β),Bcl-2-associated X protein(Bax)and cysteinyl aspartate specific proteinase-3(Caspase-3)as well as the contents of tumor necrosis factor-α(TNF-α)and interleukin-6(IL-6)were significantly higher than those of the control group;Wnt1 andβ-catenin were positively correlated with VEGF,IGF-1,bFGF and Bcl-2,and negatively correlated with TNF-α,IL-6,Bax and Caspase-3;GSK-3βwas negatively correlated with VEGF,IGF-1,bFGF,and Bcl-2,and positively correlated with TNF-α,IL-6,Bax and Caspase-3.Conclusion:Wnt/β-catenin pathway is inhibited in diabetic foot wound and it is related to the changes in cytokine secretion and apoptosis gene expression.