Effects of acupuncture on endoplasmic reticulum stress and apoptosis in CA1 region of hippocampus of rats with Alzheimer''''s disease

摘要

cqvip:Objective:To investigate the effects of acupuncture on endoplasmic reticulum stress and apoptosis in hippocampal neurons of rats with Alzheimer's disease(AD).Methods:A total of 36 Wistar rats were randomly divided into the blank group(the control),model group,sham-operated group,and acupuncture group,with 9 rats in each group.The AD was replicated by injection of beta-amyloid protein 1-42 solution into the bilateral hippocampal CA1 area in the model group and acupuncture group.The sham-operated group was injected with the same amount of sterile hydrogen peroxide while the control group was not treated.Rats in the acupuncture group were treated with cluster needling of scalp point therapy from 2 days after model establishment once a day for 14 consecutive days,while rats in the control group,model group,and sham-operated group were bundled and fixed in the dorsal position.After treatment,the morphology of hippocampal CA1 neurons was observed by HE staining,the apoptotic level of hippocampal CA1 neurons was detected by Tunel method,the expression of Abeta 1-42 and glucose-regulated protein 78(GRP78)in hippocampal CA1 region was detected by immunohistochemistry,and the levels of GRP78 and Caspase-12 proteins in hippocampal CA1 region were detected by Western blot.Results:Compared with the blank group,the neurons in the hippocampal CA1 area in the model group decreased significantly,and the apoptotic rate,the expression of Abeta 1-42,GRP78 and Caspase-12 protein increased significantly(P<0.01).Compared with the model group,the morphology of hippocampal CA1 neurons in the acupuncture group was significantly improved,the number of neurons was significantly increased,the apoptotic rate,the expression of Abeta 1-42,GRP78 and Caspase-12 protein were significantly reduced,and the acupuncture group was significantly improved(P<0.01).Conclusions:Scalp acupoint cluster needling therapy improves the morphology of hippocampal CA1 neurons and inhibits the accumulation of Abeta 1-42 by inhibiting endoplasmic reticulum stress and cell apoptosis.