Effect of small dose hormone on TLR3 expression in peripheral blood mononuclear cells of children with sepsis and its correlation with the change of the illness

摘要

cqvip:Objective:To study the effect of small dose hormone on TLR3 expression in peripheral blood mononuclear cells of children with sepsis and its correlation with the change of the illness. Methods: Patients who were diagnosed with sepsis in Yan'an People's Hospital between June 2014 and September 2017 were chosen as the research subjects and randomly divided into two groups, hormone group received small-dose glucocorticoid combined with routine therapy and control group accepted routine therapy;the TLR3 mRNA expression levels in peripheral blood as well as the contents of inflammation molecules and oxidative stress molecules in serum were measured before treatment as well as 3 d, 5 d and 7 d after treatment.Results: 3 d, 5 d and 7 d after treatment, TLR3 mRNA expression levels in peripheral blood as well as TNF-α, ICAM-1, sTREM1, Presepsin, HMGB1, ESM1, LPO and MDA contents in serum of both groups of children were lower than those before treatment whereas SOD and GSH-Px contents were higher than those before treatment, and TLR3 mRNA expression levels in peripheral blood as well as TNF-α, ICAM-1, sTREM1, Presepsin, HMGB1, ESM1, LPO and MDA contents in serum of hormone group were lower than those of control group whereas SOD and GSH-Px contents were higher than those of control group;the TLR3 mRNA expression of hormone group was positively correlated with TNF-α, ICAM-1, sTREM1, Presepsin, HMGB1, ESM1, LPO and MDA contents, and negatively correlated with SOD and GSH-Px contents.Conclusion: Small-dose hormone can inhibit the expression of TLR3 in peripheral blood mononuclear cells of children with sepsis to inhibit the inflammatory response and oxidative stress response in the course of disease.