Correlation of PRPS1 and JAB1 expression in the bone marrow tissue of acute leukemia with the apoptosis and invasion of tumor cells

摘要

Objective:To study the correlation of PRPS1 and JAB1 expression in the bone marrow tissue of acute leukemia with the apoptosis and invasion of tumor cells.Methods:Patients who were diagnosed with acute leukemia by bone marrow aspiration biopsy in Edong medical group Huangshi central hospital between February 2015 and December 2017 were selected as the AL group of the research, and patients who received bone marrow aspiration biopsy and were without significant abnormality in Edong medical group Huangshi central hospital during the same period were selected as the control group of the research. RNA was extracted to determine the mRNA expression of PRPS1, JAB1, apoptosis genes and invasion genes, and protein was extracted to determine the protein expression of PRPS1 and JAB1.Results:Both mRNA expression and protein expression of PRPS1 and JAB1 in bone marrow tissues of AL group were significantly higher than those of control group;AuroraA, Bcl-2,β-catenin, MMP2, MMP9, N-cadherin and Msi2 mRNA expression in bone marrow tissues of AL group were significantly higher than those of control group whereas Bax, C/EBP , RUNX3 and TIMP3 mRNA expression were significantly lower than those of control group, and the PRPS1 and JAB1 mRNA expression in bone marrow tissues of AL group were positively correlated with AuroraA, Bcl-2,β-catenin, MMP2, MMP9, N-cadherin and Msi2 mRNA expression, and negatively correlated with Bax, C/EBP , RUNX3 and TIMP3 mRNA expression.Conclusion:The high expression of PRPS1 and JAB1 in the bone marrow tissue of acute leukemia can inhibit cell apoptosis and promote cell invasion.