Expressions of PKCα, PKCε and PKCδ in cervical cancer and their relationships with chemosensitivity

摘要

Objective:To investigate the expressions of PKC , PKC and PKC in cervical cancer and their relationships with chemosensitivity.Methods: A total of 38 cases of patients with cervical cancer diagnosed in our hospital from November 2015 to November 2016 were selected, and the vascularization index (VI), flow index (FI) and vascular flow index (VFI) were measured by three-dimensional color Doppler ultrasound, then the expression levels of PKC , PKC and PKC mRNAs were detected by RT-PCR before and after chemotherapy. Western blot was used to detect the expression of PKC , PKC and PKC proteins in tissues, Spearman correlation analysis was performed to investigate the relationships between PKC , PKC and PKC mRNAs with chemosensitivity, Logisitic regression analysis was used to analyze the related factors affecting the sensitivity of chemotherapy.Results: After chemotherapy, the expression levels of PKC and PKC mRNAs and proteins were significantly decreased, the expressions of PKC mRNA and protein were significantly increased;the expression levels of PKC and PKC mRNAs and proteins in the effective group after chemotherapy were significantly lower than those before chemotherapy, the expressions of PKC mRNA and protein were significantly higher than those before chemotherapy;Spearman analysis showed that the expression levels of PKC and PKC mRNAs in cervical cancer tissues before chemotherapy were positively correlated with VI, FI and the percentage of residual tumor volume after chemotherapy, PKC was negatively correlated with VI, FI and residual tumor volume percentage after chemotherapy, and was not significantly correlated with VFI and tumor size before chemotherapy;Logistic regression analysis showed that PKC , PKC , PKC mRNAs, VI and FI were independent risk factors for chemosensitivity, lymph node metastasis and tumor volume were independent of chemosensitivity.Conclusion: PKC and PKC are highly expressed in cervical cancer tissues, and PKC is low expressed in cervical cancer tissues, they are closely related to chemosensitivity.