Correlation of the electrical remodeling of myocardial cells with oxidative stress and inflammatory injury in animal models with dilated cardiomyopathy

摘要

Objective:To study the correlation of the electrical remodeling of myocardial cells with oxidative stress and inflammatory injury in animal models with dilated cardiomyopathy. Methods: Male New Zealand white rabbits were selected as experimental animals and divided into model group and control group, model group were made into animal models with dilated cardiomyopathy through ear intravenous injection of adriamycin, and the control group received ear intravenous injection of same dose of saline. 8 weeks later, the myocardial tissue was collected to detect the myocardial cell delay after depolarization (DADs) through the technique of unicell patch clamp, and radioimmunoprecipitation kits and enzyme-linked immunosorbent assay kits were used to determine oxidative stress indexes and inflammatory reaction indexes.Results:The incidence of myocardial cell DADs of animal model group was significantly higher than that of control group, and ROS, MDA, AOPP, CHOP, GRP78, TLR4, NF-kB, IL-1β, IL-6 and TNF-α levels in myocardial tissue were significantly higher than those of control group;ROS, MDA, AOPP, CHOP, GRP78, TLR4, NF-kB, IL-1β, IL-6 and TNF-α levels in myocardial tissue of DADs animals in model group were significantly higher than those of non-DADs animals.Conclusion:Adriamycin-induced dilated cardiomyopathy can lead to myocardial cell delay after depolarization through oxidative stress and inflammatory injury.