Effect of p53-targeted small molecular reactivator of p53 and induction of tumor apoptosis (RITA) combined with temozolomide (TMZ) on the glioma cell growth in vitro

摘要

Objective:To study the effect of p53-targeted small molecular reactivator of p53 and induction of tumor apoptosis (RITA) combined with temozolomide (TMZ) on the glioma cell growth in vitro.Methods:Human glioma cell lines U87 were cultured and randomly divided into RITA+TMZ group (treated with 5 μmol/L RITA and 10 μmol/L TMZ), TMZ group (treated with 10 μmol/L TMZ) and control group (treated with drug-free DMEM). After 24 h of treatment, the expression of p53 downstream cell cycle molecules, apoptosis molecules and invasion molecules in cells were measured.Results:p21cip1, Per2, ATM and E-cadherin protein expression in RITA+TMZ group and TMZ group were significantly higher than those in control group while CDK4, CDK6, p-Rb, E2F, MDM2, c-myc, ILK, Snail and Slug protein expression were significantly lower than those in control group;p21cip1, Per2, ATM and E-cadherin protein expression in RITA+TMZ group were significantly higher than those in TMZ group while CDK4, CDK6, p-Rb, E2F, MDM2, c-myc, ILK, Snail and Slug protein expression were significantly lower than those in TMZ group.Conclusion:p53-targeted small molecular RITA combined with temozolomide treatment of glioma cells can induce p53-mediated cell cycle arrest, cell apoptosis activation and cell invasion inhibition.