Correlation of apoptosis with invasion and angiogenesis in decidual tissue of recurrent spontaneous abortion

摘要

Objective: To study the correlation of apoptosis with invasion and angiogenesis in decidual tissue of recurrent spontaneous abortion (RSA). Methods: Patients with recurrent spontaneous abortion who were treated in Zigong Maternity and Child Healthcare Hospital between July 2014 and March 2017 were selected as the RSA group of the research, and the pregnant women who were with spontaneous labor in Zigong Maternity and Child Healthcare Hospital during the same period and without history of abortion were selected as the control group of the research. Decidualtissue was collected to determine the mRNA expression of apoptosis genes and invasion genes as well as the protein levels of pro-angiogenesis molecules, and the serum was collected to determine the protein levels of anti-angiogenesis molecules. Results: Bcl-2 mRNA expression in decidual tissue of RSA group was significantly lower than that of control group while Bax, Caspase-3, Caspase-9 and TRAIL mRNA expression were significantly higher than those of control group;MMP2 and MMP9 mRNA expression as well as VEGF, Ang-1, Ang-2 and Tie-2 protein levels in decidual tissue of RSA group were significantly lower than those of control group, positively correlated with Bcl-2 mRNA expression, and negatively correlated with Bax, Caspase-3, Caspase-9 and TRAIL mRNA expression;TIMP1 and TIMP2 mRNA expression in decidual tissue as well as sFlt-1 protein level in serum were significantly higher than those of control group, negatively correlated with Bcl-2 mRNA expression, and positively correlated with Bax, Caspase-3, Caspase-9 and TRAIL mRNA expression. Conclusion: Pro-apoptosis gene expression increases and anti-apoptosis gene expression increases in decidual tissue of RSA, and the excessive apoptosis is related to the insufficiency of invasion and the decrease of angiogenesis.