RNF181 and TPX2 expression in colon cancer tissue and their correlation with cancer cell viability and angiogenesis

摘要

Objective:To study the RNF181 and TPX2 expression in colon cancer tissue and their correlation with cancer cell viability and angiogenesis.Methods:Colon cancer tissue and tissue adjacent to carcinoma that were surgically removed in our hospital between July 2013 and May 2016 were collected and made into paraffin sections, immunohistochemical staining for RNF181 and TPX2 were conducted, and the positive protein rate was determined;RNA was extracted for fluorescence quantitative PCR reaction, and the mRNA expression of RNF181, TPX2, VEGF, VEGFR1, VEGFR2 and HIF-1α were determined;the preoperative serum samples of colon cancer patients were collected to determine the contents of CEA, CA199 and CA242 levels.Results:mRNA expression and positive protein rate of RNF181 in colon cancer tissue were significantly lower than those in adjacent tissue while mRNA expression and positive protein rate of TPX2 were significantly higher than those in adjacent tissue;CEA, CA199 and CA242 levels in serum as well as VEGF, VEGFR1, VEGFR2 and HIF-1α mRNA expression in colon cancer tissue of patients with positive RNF181 in colon cancer tissue were significantly lower than those of patients with negative RNF181;CEA, CA199 and CA242 levels in serum as well as VEGF, VEGFR1, VEGFR2 and HIF-1α mRNA expression in colon cancer tissue of patients with positive TPX2 in colon cancer tissue were significantly higher than those of patients with negative TPX2.Conclusion: The low expression of RNF181 and high expression of TPX2 in colon cancer tissue are closely related to the cancer cell proliferation and angiogenesis process.