Eucommia and fructus psoraleae promote the proliferation of osteoblasts cultured in vitro: an experimental study

摘要

Objective:To study the effect of eucommia and fructus psoraleae treatment on the proliferation of osteoblasts cultured in vitro.Method:1-day-old SD rats were taken, the osteoblasts in exposed bone were separated, cultured and divided into 4 groups, group A were treated with DMEM without drugs or serum, group B were treated with serum-free DMEM containing eucommia with final concentration 10 μg/L, group C were treated with serum-free DMEM containing fructus psoraleae with final concentration 10 μmol/L, and group D were treated with serum-free DMEM containing eucommia with final concentration 10 μg/L and fructus psoraleae with final concentration 10 μmol/L. 24 h after treatment, the mRNA expression of apoptosis genes and proliferation genes in cells were detected.Results: Bax, Fas, FasL and HSG mRNA expression in group B, group C and group D were significantly lower than those in group A while c-fos, c-jun, CyclinD1, Egr-1 and NDRG1 mRNA expression were significantly higher than those in group A;Bax, Fas, FasL and HSG mRNA expression in group D were significantly lower than those in group B and group C, and c-fos, c-jun, CyclinD1, Egr-1 and NDRG1 mRNA expression were significantly higher than those in group B and group C.Conclusion: Both eucommia and fructus psoraleae can promote the osteoblast proliferation, and the combination of the two drugs has synergistic effect.