Differential expression of circRNA CPT1A in peripheral blood of patients with type 2 diabetes mellitus

摘要

Objective:To investigate the clinical value of circRNA CPT1A in type 2 diabetes mellitus(T2DM)by observing the relative expression levels of circRNA CPT1A in the peripheral blood of type 2 diabetic patients.Methods:Peripheral blood,as well as general data were collected from 20 type 2 diabetic patients and 20 healthy check-ups attending the First Affiliated Hospital of Shihezi University School of Medicine,and a glucose tolerance test was perfected to detect relevant biochemical indexes in peripheral blood of diabetic and healthy control groups,and the relative expression level of circRNA CPT1A was detected by qRT-PCR technique;ROC curve was used to evaluate the diagnostic value of circRNA CPT1A in T2DM;correlation analysis was used to explore the correlation between target circRNA and glucolipid metabolic indexes;logistic regression model was used to analyze the effect of circRNA CPT1A on the risk of T2DM.Results:The relative expression levels of circRNA CPT1A were higher in the T2DM group than in the healthy control group(P<0.001).ROC curves showed that circRNA CPT1A has a diagnostic value in T2DM.The correlation analysis showed that the expression level of circRNA CPT1A correlated with fasting glucose,2 h post-glucose load,HOMA-IR,HOMA-β,and triglycerides(P<0.05).logistic regression analysis showed that the relative expression level of circRNA CPT1A increased the risk of T2DM(P<0.05).Conclusion:The expression levels of circRNA CPT1A were significantly elevated in the peripheral blood of T2DM patients,suggesting that they may be a potential diagnostic marker for T2DM.