复方竹叶石膏颗粒对家兔急性放射性食管损伤Bcl-2与Bax mRNA及蛋白表达的影响

摘要

目的 探讨复方竹叶石膏颗粒对家兔急性放射性食管炎组织中Bax和Bcl-2 mRNA及蛋白表达的影响.方法 将40只雄性家兔随机分为空白对照组(A组)、单纯照射组(B组)、复方竹叶石膏颗粒组(C组)、康复新液组(D组),每组10只.B、C、D组均用6 mV-X射线对家兔食管单次照射32 Gy.C、D组于照射前1周照射至放疗结束后7d.4组均于照射后第7天留取家兔全长食管,检测Bax和Bcl-2 mRNA和蛋白的表达水平.结果 B组家兔食管组织中Bax mRNA与蛋白表达水平较A组升高,Bcl-2 mRNA与蛋白表达及Bcl-2/Bax值较A组降低(P＜0.05).C、D组Bcl-2 mRNA与蛋白表达水平及Bcl-2/Bax值高于B组,Bax mRNA与蛋白表达水平低于B组(P＜0.05).C组Bcl-2 mRNA与蛋白表达水平均高于D组(P＜0.05).结论 复方竹叶石膏颗粒能够防治急性放射性食管炎,其机制可能与上调Bcl-2基因与下调Bax基因的表达有关.%Objective To investigate effects of Compound Zhuye Shigao Granule (CZYG) on mRNA and protein expressions of bcl-2 and bax in treatment of rabbits with radiation esophagitis.Methods A total of 40 male rabbits were randomly divided into control group (n =10,group A),simple radiation group (n =10,group B),CZYG group (n =10,group C) and Kangfuxinye group (n =10,group D).All rabbits except those in control group were irradiated by 6MV X-ray at a dose of 32Gy for one time.The drugs were given starting from 1 week before the irradiation to at 7th d after irradiation in group C and D.Samples of the total length of rabbits'esophagus were collected at 7thd after irradiation in all groups to detect levels of mRNA and protein expressions of bcl-2 and bax.Results In group B,Bax mRNA and protein expressions were significantly increased,while values of Bcl-2 mRNA and protein expressions and Bcl-2/Bax were significantly decreased compared with those in group B (P ＜ 0.05).Compared with those in group B,values of Bcl-2 mRNA and protein expressions and Bcl-2/Bax were significantly increased,while Bax mRNA and protein expressions were significantly decreased in group C and D (P ＜ 0.05).Bcl-2 mRNA and protein expressions in group C were significantly increased compared with those in group D (P ＜ 0.05).Conclusion Compound Zhuye Shigao Granule (CZYG)can prevent radiation esophagitis,and its mechanism may be related to increase Bcl-2 expression and decrease Bax expression.