HPLC法测定紫果西番莲叶中异牡荆素的含量

摘要

Objective To detect the content of isovitexin in the leaves of Passiflora edulis by high performance liquid chromatography ( HPLC ). Methods Firstly, the extraction and purification were carried out by silica gel column chromatography. HPLC separation was carried out in Hypersil ODS C_18 column ( 4. 6 × 250 mm, 5μm ) with the mobile phase of acetonitrile - 0. 1% H_3PO_4 solution (18: 82 ), and the flow rate was 1. 0 ml/min. The sample size was 10 μl, and the detection wavelength was 350 nm. Results Within the range of 0.012 - 0. 120 mg/ml, isovitexin had a good linear relationship ( R =0. 997 ). The average recovery was 98. 26% , and RSD was 1. 4% . The average content of isovitexin was 0. 0873% . Conclusion The method is accurate, reliable, and repeatable, and can be used to detect the content of isovitexin in the leaves of passiflora edulis.%目的 应用HPLC法,对紫果西番莲叶中异牡荆素的含量进行测定.方法 首先采用硅胶柱色谱进行分离纯化;采用HPLC,Hypersil ODS C18色谱柱(4.6×250 mm,5 μm);乙腈-0.1磷酸(18:82)为流动相;流速1.0 ml/min;进样量:10 μl,检测波长350 nm.结果 异牡荆素在0.012～0.120 mg/ml范围内时,呈现良好线性关系(R2=0.997);平均回收率为98.26%,RSD 1.4%;紫果西番莲叶中异牡荆素的平均含量为0.0873%.结论 该方法 准确、可靠、重复性良好,可用于西番莲属植物中异牡荆素含量的测定.