甘油氧化酶法测定血清三酰甘油的研究

摘要

Objective: To establish a new glycerol oxidase determination method for serum triacylglycerol. Methods: Lipoprotein lipase, glycerol oxidase and 3 - methyl - - N - ethyl ((3 - hydroxy ethyl) aniline pigment system was applied to assay serum triacylglycerol . Results: The sensitivity had been improved to select the optimum concentration of buffer, pH and type. The linearity rage was 0 - 18mmol/L, the normal value range were from 0. 05 to 1.8 mmol/L in male, and from 0. 04 to 1. 7 mmol/L in female, the reclaimed rate was from 93% to 103 % , the precision were coefficient of variation CV = 2. 6% - 4. 2 % and CV = 3. 1 % - 4. 8 % (n = 20) , which had better associativity (7=0. 982) compared with GPO - PAP method. Conclusion: -This method has high specificity and accuracy, the operation is convenient, sensitive and speedy, the sample is less, the results ware stable and reliable, it is a new determination method for serum trglyceride.%目的:建立一种甘油氧化酶法测定血清三酰甘油新法.方法:用脂蛋白脂酶、甘油氧化酶和3 -甲基-N-乙基(β-羟乙基)苯胺(简称MEHA)色原系统进行血清三酰甘油测定.结果:对最适缓冲液浓度、pH和种类的选择,提高了灵敏度,线性范围为0～ 18 mmol/L,正常参考范围为男0.05 ～ 1.80 mmol/L、女0.04～1.70 mmol/L.其回收率为93％～103％,精密度:批内(n=20)CV=2.6％～4.2％,批间(n=20)CV=3.1％～4.8％,与酶(GPO -PAP)法比较,相关性良好(γ=0.982).结论:本法具有高度的特异性和准确性,操作简便、灵敏快速、标本用量少、结果稳定可靠,是一种新的三酰甘油测定法.