甘草酸二铵注射液相关物质含量及其测定方法研究

摘要

Objective To introduce a new method that can be used to determine the concentration of Diammonium Glycyrrhizinate Injection and its related substance.Methods The reverse phase C18 colum was used with acetonitrile -0.01 ml/L phosphoric acid solution as the mobile phase.The UV - detection wavelength was 252nm.Sample concentration was 0.5 mg/ml.Results The recovery rate of the method was 98.83％, 99.68％ and 99.47％.Conclusions This method is a reliable and accurate in determining the concentration of Diammonium Glycyrrhizinate Injection.%目的 建立高效液相色谱法测定甘草酸二铵的相关物质的含量.方法 采用反向C18柱,以0.01 mol/L磷酸溶液-乙腈(60:40)作为流动相,流速为1 ml/min,测定波长λ为252 nm,浓度为0.5 mg/ml,进样量为10 μl.结果 高效液相色谱法测定三个浓度的加样回收率分别为98.83%、99.68%、99.47%.含量测定结果 为100%.结论 采用高效液相色谱法测定甘草酸二铵注射液含量,精密度高,重组性好,结果 准确.