From July 23rd to August 15th, 2001, a field cultivation experiment was carried out to determine the limitation factors of phytoplankton in the Yangtze River estuary and the adjacent areas. The results indicated that the potential limiting nutrient was phosphorus in the Yangtze River diluted water area, nitrogen in the offshore of the Yangtze River estuary and the conversion of phosphorus to nitrogen in the middle area. Iron and silicon were not the potential limiting factors. If there were some kinds of limiting factors in the water, the growth of phytoplankton would be limited obviously. In case of disappearance of the limiting factor, the phytoplankton would grow fast. When the Noctiluca scintiuans bloom occurred, the phytoplankton biomass level was very low in a short time due to the grazing pressure. When the grazing pressure disappeared, the phytoplankton would grow quickly in abundant nutrients condition.
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