β-榄香烯诱导人黑色素瘤A375细胞凋亡的研究

摘要

β-elemene,a self-developed anticancer medicine in China,has an obvious therapeutic effect on liver cancer,lung cancer and other malignant tumors due to low toxicity,high efficacy and wide-spectrum activity,while its role on human skin cancer has not been reported.Here,the anti-tumor activity of β-elemene was investigated on human melanoma cells.It was found that the proliferation of A375 cells was significantly inhibited byβ-elemene (P＜0.01) in a concentration-and time-dependent manner.Furthermore,related apoptosis proteins were detected by Western-blot,which showed that β-elemene inhibited AKT signal pathway to regulate Bcl-2 family proteins.It reduced the expression of Bcl-2,but significantly increased the expression of Bad,and in the meantime partially activated caspase proteins,thereby inhibiting A375 cell proliferation and inducing apoptosis.%β-榄香烯是我国自主开发的二类非细胞毒性抗肿瘤药物,具有广谱、高效、低毒等优点,对肝癌、肺癌等多种恶性肿瘤均有较好的治疗效果,但对人皮肤肿瘤的研究尚无报道.基于此,就β-榄香烯对人恶性黑色素瘤A375细胞的抗肿瘤活性展开研究,发现β-榄香烯能够显著抑制A375细胞增殖(P＜0.01),并在一定范围内呈浓度和时间依赖性,主要表现为β-榄香烯能够诱导细胞发生凋亡.进一步对相关凋亡蛋白进行Western-blot检测,发现β-榄香烯通过抑制细胞内AKT信号通路途径,调控Bcl-2家族蛋白,降低Bcl-2的表达,显著增加Bad的表达量,并激活部分caspase系列蛋白质的剪切活化,从而抑制A375细胞增殖,诱导细胞凋亡.