宁夏地区耐甲氧西林金黄色葡萄球菌分子流行病学研究

摘要

目的 调查临床分离的耐甲氧西林金黄色葡萄球菌(MRSA)的染色体mec基因盒(SCCmec)分型及杀白细胞素(PVL)的pvl基因携带率,初步了解宁夏地区MRSA分子流行病学特点.方法 对2005年9月至2008年1月从临床标本中分离的MRSA菌株进行pvl基因检测,并应用多重聚合酶链反应(PCR)对MRSA菌株进行SCCmec分型.结果 88株MRSA菌株中pvl基因阳性菌株8株(9.1%),SCCmec分型结果为Ⅲ型86株(97.7%)、Ⅱ型2株(2.3%).结论 宁夏地区分离的MRSA菌株SCCmec分型以Ⅲ型为主,SCCmec分型是探索MRSA多重耐药性有效的分子生物学手段.%Objective To investigate the genotype and carry over rate of staphylococcal chromosomal cassette mec (SCCmec) and panton-valentine leukocidin (PVL) pvl gene in clinical methicillin-resistant Staphylococcus aureus (MRSA)isolates, and primarily find out the characteristic of molecular epidemiology of MRSA isolates in Ningxia.Methods The pvl gene of the MRSA strains isolated from September 2005 to January 2008 were determined, and the genotypes of SCCmec were determined by multiplex polymerase chain reaction (PCR). Results Among 88 MRSA isolates,8 isolates were pvl gene positive(9. 1％ ). SCCmec genotyping results showed that 86 isolates were Ⅲ type (97.7％), and 2 isolates were Ⅱ type ( 2.3％ ). Conclusions The most isolates of MRSA in Ningxia are SCCmec Ⅲ. SCCmec typing is an effective molecular epidemiolngy method to research the multiresistant characteristic of MRSA isolates.