粗壮女贞水提物在荷瘤裸鼠体内安全性的研究

摘要

Objective To evaluate the safety of the aqueous extract of Ligustrum robustum,(L.robustum) in tumor-bearing nude mouse.Methods Tumor-bearing nude mouse model was established,which was randomly divided into the L.Robustum group and the control group with 10 mice for each group.Mice were injected with L.robustum or intraperitoneally (2 g/kg in 100 μL of PBS) for 5 days.Body weight and mental behavior were observed.At the end of the experiment,the major organs of mice were weighted and the organs′ histological architecture were examined by HE staining.Superoxide dismutase (SOD) activity of serum was detected.Results There were no changes of body weight and mental behavior.No obvious histopathological changes of organs were observed.The superoxide dismutase (SOD) activity of serum increased significantly in L.Robustum group compared with the control group(P<0.05).Conclusion L.Robustum is safety in the tumor-bearing nude mouse model under this experimental condition.%目的 研究粗壮女贞水提物在荷瘤裸鼠体内的安全性.方法 建立裸鼠荷瘤模型,腹腔注射粗壮女贞或磷酸盐缓冲液(PBS)后,观察裸鼠体质量、精神行为、肢体活动变化;处死裸鼠后,采用脏器百分比和HE染色评价裸鼠脏器大小及其组织病理学改变;并进行血清超氧化物歧化酶(SOD)活性检测.结果 各组裸鼠未发现精神行为和肢体活动异常;体质量及脏器百分比与对照组比较,差异无统计学意义(P<0.05);HE染色显示各组裸鼠脏器组织结构均正常.与对照组比较,粗壮女贞组裸鼠血清SOD活性明显增高(P<0.05).结论 粗壮女贞水提物在荷瘤裸鼠体内具有较好的安全性,能提高其抗氧化能力.