目的 探讨原发性肝癌患者miRNA let-7a、miRNA let-7f的表达.方法 收集龙岗中心医院院及合作医院2014年6月至2016年3月接受肝癌切除手术的40例原发性肝癌患者.采用RT-PCR方法检测miRNA let-7a、miRNA let-7f在血清、肝癌/癌旁组织中的表达水平.选择同期该院体检的40例健康者作为健康对照者.比较肝癌患者和健康对照者血清miRNA let-7a、let-7f的表达差异.结果 肝癌癌变组织miRNA let-7a[(0.18±0.04)×103]、miRNA let-7f[(0.19±0.01)×103]分别较癌旁组织[(1.52±0.11)×103、(0.67±0.10)×103]低,差异有统计学意义(P<0.01);肝癌患者血清miRNA let-7a[(0.22±0.06)×103]、miRNA let-7f[(0.15±0.04)×103]较健康对照者[(1.13±0.21)×103、(0.62±0.12)×103]低,差异有统计学意义(P<0.01).结论 miRNA let-7a、miRNA let-7f在肝癌患者病变组织及血清中异常偏低表达,可作为早期诊断肝癌的重要标志物,为临床诊断提供参考.%Objective To study the expression of miRNA let-7a and let-7f in primary liver cancer patients.Methods In our hospital from June 2014 to March 2016,40 patients with primary liver cancer were sellected.the expression levels of miRNA let-7a and let-7f in serum and liver cancer/tumor adjacent tissues were detected by RT-PCR methods.A total of 40 healthy volunteers were selected at the same time and expression of miRNA let-7a,let-7f in serum were analyzed.Results The miRNA in cancer tissues [let-7a (0.18±0.04)×103,let-7f(0.19±0.01)×103]were lower than those in the adjacent tissues [let-7a(1.52±0.11)×103,let-7f(0.67±0.10)×103].The difference was statistically significant(P<0.01);In primary liver cancer,the level of miRNA let-7a[(0.22±0.06)×103,let-7f(0.15±0.04)×103] were lower than those in normal healthy people[(1.13±0.21)×103,(0.62±0.12)×103],the difference was statistically significant(P<0.01).Conclusion miRNAlet-7a,miRNA let-7f are abnormally low expression in the tissue and the serum of primary liver cancer patients.
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