目的 探讨经快速双上肺静脉起搏方法制作犬心房颤动动物模型的可行性.方法 10只健康比格犬经开胸途径植入十极导管电极,快速(1 200次/min)起搏双上肺静脉6h.观察房颤诱发情况,对起搏前后双上肺静脉及左心耳电生理检查结果进行比较.结果 8只犬完成实验,房颤诱导成功率80％.高频率刺激诱发的房颤模型能使双上肺静脉区及左心耳部位ERP先降低后升高(145±15.1 vs 130±16.3 vs 136±11.4,P=0.004、P=0.494),存在统计学显著差异.诱发房颤5h后,总的房颤诱发窗口(∑WOV)较基线略有升高,但无明显统计学差异(26.7±65.3 vs 20±52.9,P＞0.05).而在乙酰胆碱诱发房颤(第0h、1h、3h、6 h)时,可见房颤的诱发时间呈现先上升后下降的过程,而这种趋势与ERP的变化趋势存在相关性.结论 经快速双上肺起搏方法制作心房颤动动物模型,可用于房颤发病机理、电生理重构、心肌重构、分子生物学改变等临床研究,也可用于心动过速性心肌病及部分心力衰竭的研究.%Objective To evaluate the feasibility of double-superior-pulmonary veins (PVs) pacing in building atrial fibrillation(AF) model in canine.Methods After open-chest surgery,two decapolar catheter were implanted outside left-and right-superior PVs in 10 healthy Beagle dogs,rapid pacing (1 200 beat/min) were simultaneously performed for 6 hours.AF inducibility,effective refractory period (ERP) and window of vulnerability (WOV) were measured.Results AF model was successfully built in 8 canines.AF induction rate was 80％.After rapid pacing,a significant decrease and then increase could be found in ERP recording (145.±15.1 vs 130±16.3* vs 136±11.4**,*P=0.004.**P=0.494).After five hours AF inducing,the total atrial fibrillation induce window (? WOV) were increased,as compared with the baseline,but without significant difference (26.7±65.3 vs 20±52.9,P＞0.05).While in AF model induced by acetylcholine (0,1,3,6 hours),the time of atrial fibrillation induced were first rising then declining,and this trend and the correlation between the change trend of ERP.Conclusion Double superior PV pacing model can be used in pathogenesis of atrial fibrillation,electrophysiology reconstruction,myocardial reconstruction,molecular biology change clinical research,also can be used for tachycardia sex and part of heart failure cardiomyopathy.
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