AKAP3基因在被动吸烟雄鼠睾丸组织中的表达

摘要

The mature male mouse suffered by one-month,three-month and five-month passive smoking by using the established passive smoking model,comparing with normal control male mouse,the expression rule of AKAP3 in the passive smoking male mice testicular tissue was analyzed by RT-PCR. The results showed that one-month passive smoking male mouse had greater AKAP3 mRNA expression quantity than that of control male mouse. However,no significant differences were observed among three-month and five-month passive smoking male mouse. The results of this study provided theoretical basis and reference for further study of AKAP3 in male mice reproduction function.%采用前期建立的被动吸烟模型，让性成熟成年雄鼠分别接受1个月、3个月和5个月的被动吸烟，以相同年龄的正常雄鼠为对照，采用实时荧光定量 PCR 法（RT-PCR）分析 A 激酶锚定蛋白3（AKAP3）在被动吸烟雄鼠睾丸组织中的表达变化规律。结果表明：接受1个月被动吸烟雄鼠睾丸组织中 AKAP3基因 mRNA 的表达量显著高于对照组，而接受 3个月和5个月被动吸烟雄鼠睾丸组织中该基因的表达量却与对照组无显著差别，该实验结果为进一步研究 AKAP3基因对雄性生殖功能的影响机制提供了理论基础和参考。